Analysis Note

ControlK562

Application

Research CategorySignaling

Detect phospho-CrkL (Tyr207) using this Anti-phospho-CrkL (Tyr207) Antibody validated for use in WB.

Research Sub CategoryCytoskeletal Signaling

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

CRKL belongs to a family of adaptor proteins containing SH2/SH3 domains that couple to effector proteins including p130CAS, SOS, Abl, and Arg. CRKL plays a role in the mediation of Integrin- and Rac-dependent cell migration and in Ras-dependent activation of JNK. The protein is also involved in T-cell activation. Phosphorylation of CrkL on Tyr207 is known to be correlated with its inhibition.

Immunogen

KLH synthetic peptide including and encompassing Tyr 207 of human CrkL.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified rabbit polyclonal IgG in storage buffer containing PBS with 0.05% sodium azide in 50% glycerol.

Quality

Routinely evaluated by western blot on K563 cell lysates.

Specificity

Recognizes CrkL only when phosphorylated on Tyr207.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

39 kDa